(Solution) - You are trying to purify a protein that is soluble -(2025 Original AI-Free Solution)

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Academic Level: Undergrad. (yrs 3-4)

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You are trying to purify a protein that is soluble in a solution of 2 M ammonium sulfate. After centrifugation to remove other proteins that have precipitated at this high salt concentration, you recover the supernatant to assay the target protein's activity in a cell culture system.
(a) Explain why the cells die when incubated with the supernatant.
(b) What procedure could you perform to correct the problem? (Hint: See Section 2-1D).